Blue light transilluminators are usually utilized as an alternative to UV transilluminators. This occurs when users need to use “safe dyes” rather than Ethidium Bromide. They offer a “safe” light source hence the user does not come into contact with dangerous UV radiation. On the other hand, UV- transilluminators are utilized in molecular biology labs; in view of DNA or RNA that is split by electrophoresis via an agarose gel. Ethidium bromide is a poisonous chemical with limited safety protocols. Thus, it is solely advised that you use the dye in a lab with built handling, waste disposal and storage processes in place.
Performance and Adaptability
Electrical power cord is a feature in blue light transilluminators that involve adapter for linking inside nearly all of gel documentation systems. They generate brighter light and greater uniform emission comparable to UV transilluminators. The transilluminators offer maximum excitation for safe DNA gel strains and are customized for utilization with other protein and nucleic stains. Hence, there is no burn to your eyes or skin and eliminates your DNA samples upon providing improved cloning effectiveness.
Blue light transilluminators have various benefits over UV transilluminators when examining and cutting protein or DNA gels. They are securely used and do not have the risk of a burn to eyes or skin from exposure of UV. Also, there is no damage to DNA which can be a common factor when using UV transilluminators. Thus, blue light transilluminators are best selects for cutting bands from gels.
Compared to UV transilluminators, blue Light Transilluminators have several benefits when analyzing and cutting DNA or protein gels. For instance, they can be used safely and do not expose the danger of UV. More so, they have an UltraSlim blue and UltraBright light transilluminator feature which is the folding changeable position amber screen. The screen is regulated for finest viewing angles, and end-users do not need to buy and wear transilluminator glasses.
Features of Blue Light Transilluminators
These transilluminators have a high sensitivity utilizing 224 high intensity LEDs. They illuminate a broad range of samples and generate fluorescence even in faintest of bands. Its illumination has great intensity and uniform offering great quality images for analysis or publication. They have a small footprint thus are used as stand-alone transilluminators and take up small laboratory space. Generally with dimensions of 33cm x 28cm, they are easily portable to any location. More so, they are utilized with a wide range of dyes such as GelGreen, Ethidium, GelRed, SYBR Green, SYBR Gold, SYPRO Orange, SYBRSafe, SYPRO Ruby UltraSafe Blue among others.
Blue Light Transilluminators generate visible blue light that does not damage human eyes neither do it photo-nick DNA the way UV light does. Scientists can thus outlook, image and cut protein or DNA bands from gels without risking on harmful effects or their samples. They also come with a 20 x 20cm gel viewing area appropriate for many laboratory applications. These are used by scientists who require visualizing on various gels or extra huge gels.
Alan Schuster is a high school chemistry teacher, now retired. He still loves science, particularly chemistry, and blogs about the subject for a variety of sites on the web. Click here for information about how to buy chemistry lab equipment online.